LeHavreV1, Main, Exploration, bibRecord, 001537

Characterization of an inducible isoform of the Cu/Zn superoxide dismutase in the blue mussel Mytilus edulis

Identifieur interne : 001537 ( Main/Exploration ); précédent : 001536; suivant : 001538

Characterization of an inducible isoform of the Cu/Zn superoxide dismutase in the blue mussel Mytilus edulis

Auteurs : Hélène Manduzio [France] ; Tiphaine Monsinjon [France] ; Béatrice Rocher [France] ; Francois Leboulenger [France] ; Camille Galap [France]

Source :

Aquatic toxicology [ 0166-445X ] ; 2003.

RBID : Pascal:03-0325950

Descripteurs français

Pascal (Inist)
- Caractérisation, Superoxide dismutase, Antioxydant, Métalloenzyme, Cuivre, Exposition, Inducteur enzyme, Toxicité, Focalisation isoélectrique, Indicateur biologique, Variation spatiale, Port, Zone industrielle, France, Manche Mer, Mytilus edulis.
Wicri :
- geographic : France.
- topic : Cuivre.

English descriptors

KwdEn :
- Animals, Antioxidant, Biological indicator, Bivalvia (enzymology), Characterization, Copper, Copper (toxicity), English Channel, Environmental Exposure (adverse effects), Environmental Monitoring (methods), Enzyme Induction (physiology), Enzyme inducer, Exposure, France, Harbor, Industrial area, Isoelectric focusing, Isoenzymes (biosynthesis), Isoenzymes (isolation & purification), Metalloenzyme, Mytilus edulis, Oxidative Stress, Seawater (chemistry), Spatial variation, Superoxide Dismutase (biosynthesis), Superoxide Dismutase (isolation & purification), Superoxide dismutase, Toxicity, Water Pollutants, Chemical (analysis), Water Pollutants, Chemical (toxicity).
MESH :
- chemical , analysis : Water Pollutants, Chemical.
- chemical , biosynthesis : Isoenzymes, Superoxide Dismutase.
- chemical , isolation & purification : Isoenzymes, Superoxide Dismutase.
- chemical , toxicity : Copper, Water Pollutants, Chemical.
- adverse effects : Environmental Exposure.
- chemistry : Seawater.
- enzymology : Bivalvia.
- methods : Environmental Monitoring.
- physiology : Enzyme Induction.
- Animals, Oxidative Stress.

Abstract

Aerobic organisms are protected against oxidative stress by antioxidant systems which mobilise enzymes such as the Cu/Zn superoxide dismutase (Cu/Zn-SOD) which transfers O^-.₂ to H₂O₂. In this paper, we report the characterization of three isoforms of Cu/Zn-SOD in the blue mussel Mytilus edulis and we show that one of these isoforms is strongly inducible. Cytosolic extracts of digestive gland and gills from adult blue mussels were analysed by polyacrylamide gel electrophoresis or isoelectric focusing followed by in situ staining for SOD activity. Two main bands of Cu/Zn-SOD were obtained at pI 4.7 and 4.6 corresponding to native apparent molecular weight values of 205 and 155 kDa. Blue mussels from chemically contaminated area in Le Havre harbour exhibited a third Cu/Zn-SOD isoform characterized by a more acidic isoelectric point (pi 4.55) and a native apparent molecular weight of 130 kDa. When maintained in clean marine water, mussels from this area showed a transitory decrease in total SOD activity accompanied by the disappearance of the SOD-3 band. Conversely, the exposure (4 and 8 h, and 3 and 7 days) of control blue mussels to copper (25 μg 1^-1 markedly increased SOD-3 band while the total SOD activity did not systematically change. Taken together our results suggest that the variations of SOD expression pattern in Mytilus edulis could be used as a tool for the marine environment monitoring.

Affiliations:

Le document en format XML

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<term>Antioxidant</term>
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<term>Characterization</term>
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<front><div type="abstract" xml:lang="en">Aerobic organisms are protected against oxidative stress by antioxidant systems which mobilise enzymes such as the Cu/Zn superoxide dismutase (Cu/Zn-SOD) which transfers O<sup>-.</sup>
<sub>2</sub>
 to H<sub>2</sub>
O<sub>2</sub>
. In this paper, we report the characterization of three isoforms of Cu/Zn-SOD in the blue mussel Mytilus edulis and we show that one of these isoforms is strongly inducible. Cytosolic extracts of digestive gland and gills from adult blue mussels were analysed by polyacrylamide gel electrophoresis or isoelectric focusing followed by in situ staining for SOD activity. Two main bands of Cu/Zn-SOD were obtained at pI 4.7 and 4.6 corresponding to native apparent molecular weight values of 205 and 155 kDa. Blue mussels from chemically contaminated area in Le Havre harbour exhibited a third Cu/Zn-SOD isoform characterized by a more acidic isoelectric point (pi 4.55) and a native apparent molecular weight of 130 kDa. When maintained in clean marine water, mussels from this area showed a transitory decrease in total SOD activity accompanied by the disappearance of the SOD-3 band. Conversely, the exposure (4 and 8 h, and 3 and 7 days) of control blue mussels to copper (25 μg 1<sup>-1</sup>
 markedly increased SOD-3 band while the total SOD activity did not systematically change. Taken together our results suggest that the variations of SOD expression pattern in Mytilus edulis could be used as a tool for the marine environment monitoring.</div>
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Characterization of an inducible isoform of the Cu/Zn superoxide dismutase in the blue mussel Mytilus edulis

Characterization of an inducible isoform of the Cu/Zn superoxide dismutase in the blue mussel Mytilus edulis

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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